Epigenetic changes in plants

in Wien

Our group is interested in epigenetic diversity in polyploid plants, in different ecotypes and after exposure to abiotic stress. We study these aspects in Arabidopsis thaliana with well-established genetic, cytological and molecular methods, using mutants, reporter genes, chromatin analysis, flow sorting, fluorescence in situ hybridisation, defined stress treatments, specific and genome-wide expression assays and bioinformatic approaches.
http://www.gmi.oeaw.ac.at/en/research/research-groups/ortrun-mittelsten-scheid/

Technologies

Genetische und molekulare Analyse von Mutanten, DNA Methýlierung-Assays, Chromatin-Immunopräzipitation, Inhibitor-Experimente, Immunostaining und FISH, qRT-PCR, transcriptomics

Publications

Most relevant publications
• Baubec, T., Dinh, H.Q., Pecinka, A., Rakic, B., Rozhon, W., Wohlrab, B., von Haeseler, A., Mittelsten Scheid, O. (2010) Cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic states in Arabidopsis. Plant Cell 22: 34-47
• Foerster, A.M., Mittelsten Scheid, O. (2010) Analysis of DNA methylation in plants by bisulfite sequencing. Methods Mol. Biol. 631: 1-11

(…)

Contact

Dr. Ortrun MittelstenScheid
Gregor Mendel Institute of Molecular Plant Biology
Dr. Bohr-Gasse 3
A-1030 Vienna
Austria

Phone: +43 1 79044 9830

Email: